Protein Expression And Purification Related Services

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Custom Protein Purification, E. Coli

For protein expression and purification in E.coli, customer has five different tags for selecting: GST, MBP, His, Flag, biotin. Customer could choose N terminal or C terminal insert of the tag. We also provide tag remove and endotoxin removal services.

  • Cloning
  • Subcloning
  • Generation of bacmid DNA
  • Multiprotein Complex Expressions
  • Transfection
  • Tittering and amplification of virus
  • Optimization of the expression conditions
  • Mini- or large-scale infection
  • Conventional purification
  • Affinity purification
  • Dialysis in a proper buffer and measure of concentration
  • SDS-PAGE and Western blot analysis
  • Protocol optimization


  • Time Savings: Reduce turn around time associated with pre-clinical development. Extend the capabilities of your research.
  • Excellence: Our protein expression and purification experience ensures product quality and accuracy.
  • Cost: Custom service, rapid turnaround at cost effective prices.

ProteinOne Capabilities

With protein production and purification capacities from 1mg to over 100mg, ProteinOne can accommodate projects ranging in size from bench top to bioreactor. From target discovery through pre-clinical development and beyond, ProteinOne is the best source for high quality protein production, outstanding service, and reputation that has made ProteinOne a market leader.

Please contact us for a quote or further information:

Call at (301) 738-0782
Fax at (301) 738-0781
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