Multiplexed arrays on Nitrocellulose membranes for high throughput interaction profiling of Protein-Protein, Protein-DNA, Protein-RNA and Protein-Ligand reactions with 36 highly pure, activated Transcription factors. This plaform has full-lenght protein targets with entire coding sequences which more accurately identifies the true protein binding affinities and activities. The platform format allows for individual stock protein follow up from the company protein library for experimental confirmation. Signals are currently visualized by radioactive detection to maintain a low signal to noise ratio.