ñ  Clone gene of interest directly into an adenovirus vector.

ñ  Produce crude adenovirus stock.

ñ  Large scale amplification, adenovirus purification by CsCl banding (ultracentrifugation) and FPLC.

ñ  Titer determination.

ñ  RCA assay.

ñ  Protein production in suspension culture cells.

ñ  Production of high capacity helper-dependent (gut-less) adenovirus.

ñ Fast and Reliable: Transfer GOI to shuttle vector in 1 hour using either Gateway LR Clonase or Cre recombinase.  Generate recombinant crude adenovirus stock in 5 ~ 10 days. 2 weeks turnaround time for large scale amplification and purification.

ñ RCA-Free: Special cell lines are being used to produce high quality replication-competent-free adenovirus.

ñ Strong CMV5 promoter: Exceptional high level protein expression using CMV5-intron promoter in most cell types including dividing and non-dividing or primary cells.

ñ High Titer: Up to 1013 particles/ml or 1012 PFU/ml.

ñ High Purity: Adenovirus purification by CsCl ultracentrifugation and FPLC.

ñ High Capacity Vector: Reduce inflammation and prolonged high-level transgene expression; GOI and promoter combined can be larger than 8 kb; mutiple genes can be expressed together in one vector.

Please call for price.

Tel: 301-738-0782

Email: info@proteinone.com