Anti -RNA Pol II (anti-RPB3) antibody

 In human, RPB3 is encoded by the POLR2C gene (1). This gene encodes the third largest subunit of RNA polymerase II.  The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase.  

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft.  In the pol II complex, RPB3 interacts with RPB5 and the RPB3–RPB5 interaction is intensified in the presence of three subunits, RPB7, RPB8 and RPB11. Besides, RPB3 also interacts with RPB1-5, 7, 10-12 (2).

RPB3 is involved in tissue-specific transcription and muscle differentiation via interaction with the myogenic factor Myogenin (3). RPB3 also interact with IGFBP3 (4).

Each vial contains 100 mg IgG in 0.1 ml of PBS.

Specificity
α-p33 reacts with the p33 subunit of the RNA pol II complex in HeLa nuclear extract by Western Blotting. Recommended dilution range for Western blot analysis: 1:2000-1:5000. Recommended starting dilution: 1:2000.

Storage
Aliquot and store at –20°C.
 
References:
1. Woychik N.A., et al.,(1990) Genes Dev 4, 313-323
2. Kim, T.K., et al., (1997) Proc. Natl. Acad. Sci. USA 94, 12268-12273
3. Lin, Y., et al., (1997) J. Biol. Chem. 272, 7132-7139
4. Wei, W., et al., (2001) J. Biol. Chem. 276, 12266-12273
5. Dorjsuren, D., et al., (1998) Mol Cell Biol 18, 7546-7555
6. Miyao T., et al., (1996) Genes Cells 1, 843-584
7. Kimura, M., et al., (2000) Nucleic Acids Res 15, 952-959