HMG-1 antibody

High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein (1). Mammalian HMG1 has two homologous DNA-binding domains HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues (2). HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction or unwinding, or by direct contacts with distinct cellular proteins (3-5). HMG-1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation (6, 7) or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences (8, 9). Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release (10).

Each vial contains 0.25 ml of anti-serum.

Specificity
α-HMG-1 reacts with the HMG-1 protein in HeLa nuclear extract by Western Blotting. Recommended dilution range for Western blot analysis: 1:2000-1:5000. Recommended starting dilution: 1:2000.

Storage
Aliquot and store at –20°C.
 
References:
1. Bianchi, M.E., et al., (1989) Science 243, 1056-1059
2. Bustin, M., et al., (1990) Biochem. Biophys. Acta 1049, 231-243
3. Zappavigna, V., et al., (1996) EMBO J. 15, 4981-4991
4. Ge, H., et al., (1994) J. Biol. Chem. 269, 17136-17140
5. Zlatanova, Y. et al., (1998) FASEB J. 12, 421-431
6. Stelzer, G., et al., (1994) Mol. Cell. Biol. 14, 4712-4721
7. Lu, W., et al., J Biol Chem 2000 Nov 10;275(45):35006-12
8. Jayaraman, L., et al., (1998) Genes Dev. 12, 462-472
9. Onate, S., et al., (1994) Mol. Cell. Biol. 14, 3376-3391
10. Yang, H., et al., (2001) Shock 15, 247-253