BRCA1 (Tumor Suppressor Protein and Transcription Factor)

The human BRCA1, the product of breast and ovarian cancer gene 1, is a hyperphosphorylated protein functioning as a tumor suppressor involved in both transcription regulation and DNA repair. BRCA1 associates with RAD51 and has shown to be required for transcription-coupled repair of DNA damage as well as for the repair of chromosomal double-strand breaks (1-5). Association with a human SWI/SNF-related complex has recently suggested a potential role of BRCA1 in linking chromatin remodeling to breast cancer (6).

Recombinant BRCA1 can be used for: 1) in vitro function studies including transcription and DNA repair; 2) protein-protein interaction assay; and 3) cell growth assay.

Purified protein is greater than 95% homogeneous based on SDS-PAGE gel analysis.
1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Concentration is variable in different lots

Supplied in 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
 
References:
1. Maldonado, E. et al., (1996) Nature 381, 86-89
2. Scully, R. et al., (1997) Proc. Natl. Acad. Sci. USA 94, 5605-5610
3. Sharan, SK. et al., (1997) Nature 386, 804-810
4. Wang, Y. et al., (2000) Genes & Dev. 14, 927-939
5. Cantor, SB. et al., (2001) Cell 105, 149-160
6. Bochar, DA. et al., (2000) Cell 102, 257-265