| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified BCL10 is greater than 90% homogeneous based on SDS-PAGE analysis. | | Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. | | Applications | Recombinant Bcl-10 protein can be used for: 1) protein-protein interaction assay; 2) in vitro transcription assay; 3) cell growth assay. | | Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. | | Synonym | c-E10; CARMEN; CIPER; CLAP; mE10 and B-cell CLL/lymphoma 10. | | Protein Sequence | MEPTAPSLTE EDLTEVKKDA LENLRVYLCE KIIAERHFDH LRAKKILSRE DTEEISCRTS
SRKRAGKLLD YLQENPKGLD TLVESIRREK TQNFLIQKIT DEVLKLRNIK LEHLKGLKCS
SCEPFPDGAT NNLSRSNSDE SNFSEKLRAS TVMYHPEGES STTPFFSTNS SLNLPVLEVG
RTENTIFSST TLPRPGDPGA PPLPPDLQLE EEGTCANSSE MFLPLRSRTV SRQ | | Background | Bcl-10 is an intracellular signaling protein identified from the t(1;14)(p22;q32) breakpoint in MALT lymphomas. The human Bcl10 gene encodes a protein of 233 amino acids containing an N-terminal caspase recruitment domain (CARD) that mediates self-oligomerization and the C-terminal region of Bcl-10 (1-3). Bcl 10 is expressed ubiquitously with high expression levels in lymphoid tissues and in the developing central nerve system (4-6). Mutations have been found in cases of follicular lymphoma and diffuse large B cell lymphoma (7). Bcl-10 is a positive regulator of antigen-receptor induced NF-kB activation and Bcl-10-initiated activation of NF-kB can be inhibited by cotransfection of dominant-negative mutants of TRAF2, NIK, IKKa or IkBa (3-5, 8). | | References | 1. Willis, T. G. et al., (1999) Cell 96, 35-45
2. Zhang, Q. et al., (1999) Nat. Genet. 22, 63-68
3. Srinivasula, S.M. et al., (1999) J. Biol. Chem. 274, 17946-17954
4. Costanzo, A. et al., (1999) J. Biol. Chem. 274, 20127-20132
5. Koseki,T. et al., (1999) J. Biol. Chem. 274, 9955-9961
6. Thome, M. et al., (1999) J. Biol. Chem. 9962-9968
7. Du, M.Q. et al., (2000) Blood 95, 3885-3890
8. Ruland, J. et al., (2001) Cell 104, 33-42 |
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