FRA-1 (Fos-related antigen 1)

 The fos-related antigen 1 (Fra-1, or FosL1: Fos-like antigen 1) is a member of the Fos onco-protein family and a component of AP-1 transcription factor (1). Similar to other members of the family, Fra-1 contains a leucine zipper domain and a highly conserved C-terminal region (2). Transcription of Fra-1 gene is regulated by multiple cis-elements and 12-O-tetradecanoylphorbol-13-acetate (TPA) (3). Fra-1 expression is associated with proliferation and invasiveness of breast cancer cells and other cancer cells (4,5).

FRA-1 can be applied in in vitro transcription assays, DNA-protein and protein-protein interaction assays.

The purified recombinant protein is greater than 95% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 20-200ng is sufficient for an in vitro transcription assay and 100ng is sufficient for a protein-protein interaction assay.
variable in different lots

Provided in 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
 
References:
1. Cohen DR, et al., (1989) Genes Dev. Feb;3(2):173-84.
2. Matsui M, et al., (1990) Oncogene. Mar;5(3):249-55.
3. Adiseshaiah P, et al., (2003) J Biol Chem. Nov 28;278(48):47423-33
4. Belguise K, et al., (2005) Oncogene. Feb 17;24(8):1434-44.
5. Wang HL, et al., (2002) Int J Cancer. Oct 1;101(4):301-10.