| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified FRA-1 is greater than 95% homogeneous based on SDS-PAGE analysis. |
| Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. 20-200ng is sufficient for an in vitro transcription assay and 100ng is sufficient for a protein-protein interaction assay. |
| Applications | FRA-1 can be applied in in vitro transcription assays, DNA-protein and protein-protein interaction assays. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Synonym | FRA; fra-1; FRA1 and FOS-like antigen 1. |
| Protein Sequence | MFRDFGEPGP SSGNGGGYGG PAQPPAAAQA AQQKFHLVPS INTMSGSQEL QWMVQPHFLG
PSSYPRPLTY PQYSPPQPRP GVIRALGPPP GVRRRPCEQI SPEEEERRRV RRERNKLAAA
KCRNRRKELT DFLQAETDKL EDEKSGLQRE IEELQKQKER LELVLEAHRP ICKIPEGAKE
GDTGSTSGTS SPPAPCRPVP CISLSPGPVL EPEALHTPTL MTTPSLTPFT PSLVFTYPST
PEPCASAHRK SSSSSGDPSS DPLGSPTLLA L |
| Background | The fos-related antigen 1 (Fra-1, or FosL1: Fos-like antigen 1) is a member of the Fos onco-protein family and a component of AP-1 transcription factor (1). Similar to other members of the family, Fra-1 contains a leucine zipper domain and a highly conserved C-terminal region (2). Transcription of Fra-1 gene is regulated by multiple cis-elements and 12-O-tetradecanoylphorbol-13-acetate (TPA) (3). Fra-1 expression is associated with proliferation and invasiveness of breast cancer cells and other cancer cells (4,5). |
| References | 1. Cohen DR, et al., (1989) Genes Dev. Feb;3(2):173-84.
2. Matsui M, et al., (1990) Oncogene. Mar;5(3):249-55.
3. Adiseshaiah P, et al., (2003) J Biol Chem. Nov 28;278(48):47423-33
4. Belguise K, et al., (2005) Oncogene. Feb 17;24(8):1434-44.
5. Wang HL, et al., (2002) Int J Cancer. Oct 1;101(4):301-10. |
