| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified ERK2 is greater than 95% homogeneous based on SDS-PAGE analysis. |
| Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. |
| Applications | Recombinant ERK2 can be used for in vitro phosphorylation, dephosphorylation assays and inhibitor screening assay. For Research Purposes Only. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Synonym | ERK; ERK2; ERT1; MAPK2; p38; p40; p41; p41mapk; P42MAPK amd MAPK1. |
| Protein Sequence | MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE
HQTYCQRTLR EIKILLRSRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH
LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLEICDFGL ARVADPDHDH
TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI
LGILGSPSQE DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK
RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS |
| Background | The extracellular signal-regulated kinase 2 (ERK2, 42kDa), also known as mitogen-activated protein kinase 1 (MAPK1), is located in an integration point of the mitogen-activated protein kinase (MAPK) cascading pathways and have been implicated in the regulation of diverse cellular processes such as proliferation, differentiation, transcription regulation and development (1-4). Activation and inactivation of ERK2, a serine/threonine protein kinase, are tightly regulated by an upstream protein kinase MEK1/2 and dual-specificity protein phosphatases (DSUP). In human cancers and human disorders, aberrantly upregulated MEK1 activates its unique substrate ERK1/2. Therefore, the Ras-Raf-MEK1-ERK2 pathway has become an attractive target for anticancer therapeutics (5). |
| References | 1. Meloche S and Pouysségur J (2007) Oncogene 26, 3227–3239
2. Yoon S and Seger R (2006) Growth factors 24, 21-44
3. Coleman ML and Marshall CJ (2001) Nat Cell Biol 3. E250 - E251
4. Rossomando AJ etal., (1992) PNAS 89, 5779-5783
5. Sebolt Leopold JS and Herrera R (2004) Nat Rev Cancer 4, 937-947 |
