Custom Protein Purification, E. coli

Custom Protein Purification
Our Custom Protein Purification offers all aspects of protein expression, production, purification, and development, from gene to protein including:

• Cloning
• Subcloning(as least four different tags for selection)
• Optimization of the expression conditions(three different bacterial strains for comparison)
• Mini- to large-scale cultures (step wise scale up)
• Conventional purification (Q, SP, DEAE, heparin, HAT combined with FPLC system)
• Affinity purification (His-tag, Flag tag, GST-tag, MBP-tag)
• Dialysis in a proper buffer and measure of concentration
• Lyophilization optimize
• SDS-PAGE and Western blot analysis
• Protocol optimization

Benefits

• Time Savings: Reduce turn around time associated with pre-clinical development. Extend the capabilities of your research.
• Excellence: Our protein expression and purification experience ensures product quality and accuracy.
• Cost: Custom service, rapid turnaround at cost effective prices.

ProteinOne Capabilities
With protein production and purification capacities from 1mg to over 100mg, ProteinOne can accommodate projects ranging in size from bench top to bioreactor. From target discovery through pre-clinical development and beyond, ProteinOne is the best source for high quality protein production, outstanding service, and reputation that has made ProteinOne a market leader.


Please contact us for a quote or further information:

Call at (301) 738-0782

Fax at (301) 738-0781

Or e-mail at info@proteinone.com