GST-TFIIA-p55

The transcription factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (α subunit), 19 kDa (β subunit) and 12 kDa (γ subunit). The α and β subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (γ subunit), retains native TFIIA activity (1-4).

Synonym : MGC129969; MGC129970; TF2A1; TFIIA; Homo sapiens general transcription factor IIA, 1, 19/37kDa(GTF2A1).

GST-p55 subunit of TFIIA is isolated from a strain of E. coli that contains the coding sequence of human p55 of TFIIA under the control of a T7 promoter (5).
GST-TFIIA can be used to study protein-protein interactions (6).
GST-p55 is greater than 95% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 100 units (ng) are sufficient for protein-protein interaction assays.
variable in different lots
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
 
References:
1. Buratowski, S. et al., (1989) Cell 56, 549-561
2. Ranish, J. et al., (1991) J. Biol. Chem. 266, 19320-19327
3. DeJong, J. et al., (1993) Genes & Dev. 7, 2220-2234
4. Ma, D. et al., (1993) Genes & Dev. 7, 2246-2257
5. Sun, X. et al., (1994) Genes & Dev. 8, 2336-2348
6. Kaludov, N., et al., (2000) Nucleic Acids Res. 28, 1921-1928