GST-TFIIB

The transcription factor IIB (TFIIB) is an essential factor for transcription by RNA polymerase II. TFIIB has been shown to be required for selective binding of RNA polymerase II, TFIIF and TFIID-DNA complex, and for specifying the start site of transcription. Human TFIIB is a single, 35 kDa polypeptide, homologous to yeast factor , the product of the SUA7 gene (1-4).
Isolated from an E. coli strain that carries the coding sequence for human TFIIB under the control of T7 promoter (5).
GST-TFIIB has been used for protein-protein interactions assay (6).
The purified protein is greater than 95% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay detected by an immuno-blot system.
variable in different lots
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
 
References:
1. Malik, S. et al., (1991) Proc. Natl. Acad. Sci. USA 88, 9553-9557
2. Conaway, R. et al., (1993) Annu. Rev. Biochem. 62, 161-190
3. Orphanides, G. et al., (1996) Genes & Dev. 10, 2657-2683
4. Buratowski, S. et al., (1989) Cell 56, 549-561
5. Ge, H. et al., (1996) Methods Enzymol. 274, 57-71
6. Kaludov, N., et al., (2000) Nucleic Acids Res. 28, 1921-1928