GST-RPB12

RPB12 is shared between all three RNA polymerases (1) and is homologous to the archaeal P subunit of RNA polymerase (2). Like RPB10, it is essential for yeast cell viability (3). RPB12 is also a zinc-binding protein with a canonical tetra-coordinating zinc motif but binds zinc in vitro much less efficiently than the RPB10 subunit (4). It has been shown to interact with both RPB1 and RPB2 in vitro (5) and the human subunit can functionally substitute for its yeast counterpart (6).

Recombinant RPB12 is isolated from an E. coli strain that carries the coding sequence of human RPB9 under the control of a T7 promoter.
hRPB12 has been applied in protein-protein interactions assays.
Protein is greater than 95% homogeneous based on SDS-PAGE analysis.
100 units (equal 100 ng) are sufficient for a protein-protein interactions assay.
variable in different lots
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
 
References:
1. Woychik, N.A., et al., (1994), Raven Press, In Transcription: Mechanisms and Regulation, Conway&Conway eds., 227-242
2. Werner, F., et al., (2000), Nucleic Acids Res. 28, 4299-4305
3. Woychik, N.A., et al., (1990) J. Biol. Chem. 265, 17816-17819
4. Carles, C., et al., (1991) J. Biol. Chem. 266, 24092-24096
5. Ishiguro, A., et al., (1998) J. Mol. Biol. 279, 703-712
6. McKune, K., et al., (1995) Mol. Cell. Biol. 15, 6895-6900