GST-CTF1

The CCAAT-box binding protein 1 (CTF1, also called NF-I or NF-1) is a proline-rich transcription activator (1,2). It selectively activates transcription of genes that contain GCCAAT consensus sequence in the promoter region, including human Ha-Ras, alpha-globin, hsp70 and many other cellular and viral genes (1, 3-5). Cloning and functional characterization of the protein revealed that CTF1 contains a N-terminal DNA binding domain and a C-terminal proline-rich activation domain (2,3). CTF1 was shown to interact with the general transcription factor IIB (TFIIB) and facilitate the recruitment of TFIIB to TBP-DNA complexes (6), with the forkhead thyroid transcription factor TTF2 to regulate hormone–dependent transcription of thyroperoxidase gene (7), as well as other receptor proteins to synergize hormone receptor-responsive activation (8-10).

The proline-rich activation domain of human CTF1 protein (amino acids 399-499) is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter.
GST-CTF1 has been used for protein-protein interaction assays.
GST-CTF1 protein is greater than 90% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay.
variable in different lots
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
 
References:
1. Johns, KA. et al., (1987) Cell 48, 79-89
2. Santoro, C. et al., (1988) Nature 334, 218-224
3. Mermod, N. et al., (1989) Cell 58, 741-753
4. Lum, LS et al., (1990) Mol. Cell. Biol. 10, 6709-6717
5. Cardinaux, JR. et al., (1994) J Biol. Chem. 269, 32947-32956
6. Kim, TK. et al., (1994) Proc. Natl. Acad. Sci. USA 91, 4170-4174
7. Oritz, L. et al., (1999) J Biol. Chem. 274, 15213-15221
8. Corthesy, B. et al., (1989) Mol. Cell. Biol. 9, 548-5562
9. Toohey, MG. et al., (1990) J Viol. 64, 4477-4488
10. Candeliere, AG. et al., (1996) Mol. Cell. Biol. 16, 584-592