GST-LXR-alpha-LBD

Liver X receptors (LXRs) are nuclear receptors that regulate the metabolism of cholesterol and bile acids (1). There are two subtypes of LXRs, LXRα and LXRβ. LXRα is preferentially expressed in liver, small intestine, kidney and spleen (2, 3). In contrast, LXRβ expression is ubiquitous (4). The genomic structure and the promoter regions of the two LXR genes contain specific regulatory sites, which suggest that LXRs may have physiological roles in the immune system (5). Like other nuclear receptors, LXRs heterodimerize with retinoid X receptor (RXR) for function (1). LXRs are activated by naturally occurring oxysterols and regulate the expression of target genes (6-8), including ATP binding cassette transporter 1 (ABCA1), ATP binding cassette transporter 8 (ABCG8) and cholesterol ester transfer protein (CETP) (9-10). The ligand binding domain (LBD) of LXR determines ligand specificity (11) and mediates the trans-activation function of the receptor (12).
Recombinant GST-LXRα-LBD is isolated from an E. coli strain that carries the coding sequence of the human LXRα under the control of a T7 promoter.
GST-LXRα-LBD can be applied in DNA and protein-protein interaction assays.
The purified recombinant protein is greater than 95% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interactions assay.
variable in different lots
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
 
References:
1. Peet et al., (1998) Curr. Opin. Gent. Dev. 8, 571-575
2. Willy et al., (1995) Genes Dev. 9, 1033-1045
3. Apfel et al., (1994) Mol. Cell. Biol. 14, 7025-7035
4. Song et al., (1994) Proc. Natl. Acad. Sci. 91, 10809-10813
5. Alberti et al., (2000) Gene 243, 93-103
6. Janowski et al., (1996) Nature 383, 728-731
7. Lehmann et al., (1997) J. Biol. Chem. 272, 3137-3140
8. Janowski et al., (1999) Proc. Natl. Acad. Sci. 96, 266-271
9. Luo et al., (2000) J. Clin. Invest. 105, 513-520
10. Venkateswaran et al., (2000) J. Biol. Chem. 275, 14700-14707
11. Renaud et al., (1995) Nature 378, 681-689
12. Bourguet et al., (1995) Nature 375, 377-382