GST-USF1

Human Upstream Stimulatory Factor 1 (USF1) regulates the transcription of many genes involved in lipid and glucose homeostasis and colocalizes with familial combined hyperlipidemia (FCHL) and type 2 diabetes on chromosome 1q22-23. (1-3). The USF1 protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. It is a basic helix-loop-helix leucine zipper (b-HLH-ZIP) protein that shares a common DNA-binding specificity with the c-Myc oncoproteins (4-6).
Recombinant human USF1 is isolated from an E. coli strain that carries the coding sequence of the human USF1 under the control of a T7 promoter.

Synonym: bHLHb11; FCHL; FCHL1; HYPLIP1; MLTF; MLTFI and UEF.

Recombinant USF1 can be used for: 1) in vitro transcription assays; 2) DNA binding assays (gel mobility shift assys and DNA footprint assays), and 3) protein-protein interaction assays.
The purified recombinant protein is greater than 95% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 5-50 units are sufficient for the DNA binding assays. 50-500 units are required for in vitro transcription assay and 100 units are required for protein-protein interaction assays.
variable in different lots
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
 
References:
1.Putt, W. et al., (2004) Hum. Mol. Genet. 13, 1587-1597.
2.Pajukanta, P. et al., (2004) Nat. Genetics 36, 371-376.
3.Du, H. et al., (1993) EMBO J. 12, 501-511.
4.Gregor, PD. Et al., (1990) Genes & Dev. 4, 1730-1740.
5.Ge, Y. et al., (2003) J. Biol. Chem. 278, 49901-49910.
6.Sawadogo, M. et al., (1985) Cell 43, 165-175.