GST-SRC1-RID [627-786]

Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivoator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family (1). SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction (2). The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors (3,4) and has been previously used in assays detecting ligand-dependent receptor-cofactor interactions (5).
The wild type human GST-SRC1 (amino acids 627-786) was expressed in e.Coli and purified by an affinity column.
Research Use Only and not for Diagnostic purposes. Recombinant GST-SCR1 (627-786) can be used for protein-protein interaction assays.
Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase and RNase activity.
1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay.
0.5 mg/ml (in 1X dilution buffer A).
Buffer A: Tris-Cl , Glycerol, KCl, EDTA and DTT
 
References:
1. Onate, S. et al., (1995) Science 270, 1354-1357
2. Heery, D. et al., (1997) Nature 387, 733-736
3. Xu, J. and Li, Q., (2003) Mol. Endocrinol. 17, 1681-1692
4. Bai, Y. and Giguere, V., (2003) Mol. Endocrinol. 17, 589-599
5. Cho, M. et al., (2003) J. Biochem. Mol. Biol. 36, 207-213