GST-LRH-1 (NR5R2)

LRH-1 (NR5A2) is expressed specifically in pancreas and liver, playing important roles in the regulation of several liver-specific genes.(1) LRH-1 is a mammalian homologue of Drosophila fushi tarazu factor (dFTZ-F1) and structurally belongs to the orphan nuclear receptor superfamily. LRH-1 can recognize the DNA sequence 5`-AAGGTCA-3`, the canonical recognition motif for steroidogenic factor 1 (SF-1).(2) Enhancer II (ENII) is one of the critical cis-elements in the Hepatitis B Virus (HBV) genome for the hepatic viral gene transcription and DNA replication. The liver-specific activity of ENII is regulated by multiple liver-enriched transcription factors, including LRH-1/hB1F. (3) The role LRH-1 and dosage-sensitive sex reversal, adrenal hypoplasia congenital critical region on the X chromosome, gene 1 (DAX-1) in the regulation of StAR gene expression in human granulosa cell tumor cells has been investigated. (4) LRH-1, is expressed in granulosa cells and has been shown to synergize with the cAMP signaling system to regulate the gonadal type II aromatase promoter in transient transfection assays. Observations support a direct role for LRH-1 in the induction of the progesterone but not the estrogen biosynthetic pathway during granulosa cell differentiation. (5) The human nuclear receptor liver receptor homolog 1 (hLRH-1) plays an important role in the development of breast carcinomas. results indicate that hLRH-1`s control of gene expression is mediated by phospholipid binding, and establish hLRH-1 as a novel target for compounds designed to slow breast cancer development.(6)

Synonym include:  Homo sapiens nuclear receptor subfamily 5, group A, member 2 (NR5A2), transcript variant 2; B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; hB1F; hB1F-2; LRH-1 and LRH1.

Recombinant LRH-1 is isolated from an E coli strain that carries the coding sequence of the human LRH-1 under the control of a T7 promoter and purified by using an affinity column and FPLC chromatography.
Research Use Only, not for Drug or Diagnostic purposes. Purified LRH-1 can be used for DNA, Protein-Protein interaction, and gel mobility shift assays.
Purified LRH-1 protein is greater than 95% homogeneous and contains no detectable protease, DNase, and RNase activity. The protein was further confirmed by Western Blot analysis using a polyclonal antibody.
1 unit equal 1 nanogram.
0.5 mg/ml (in 1X dilution buffer A)
Buffer A: Tris-Cl ,Glycerol, KCl, DTT, EDTA.
 
References:
1. Zhang CK et al., Gene. 2001 Aug 8;273(2):239-49.
2. Wang ZN et al., J Mol Endocrinol. 2001 Oct;27(2):255-8.
3. Cai YN et al., Cell Res. 2003 Dec;13(6):451-8.
4. Kim JW et al., J Clin Endocrinol Metab. 2004 Jun;89(6):3042-7.
5. Saxena D, et al., Endocrinology. 2004 Aug;145(8):3821-9. Epub 2004 Apr 29.
6. Ortlund EA, et al., Nat Struct Mol Biol. 2005 Apr;12(4):357-63.