GST-CAR

The constitutive androstane receptor (CAR) was identified as a member of the orphan nuclear hormone receptor family in 1994 (1). Although constitutively acitve, it can be further activated by `phenobarbitol-like` compounds, the most potent being the synthetic compound TCPOBOP (2). Upon activation, CAR regulates the xenobiotic drug metabolizing enzymes, cytochrome P450s (2). There are several overlapping functions between the nuclear receptors PXR and CAR (3). Recently, CAR, as well as PXR, have been shown to place a role in bile acid clearance and cholestatic liver injury (4,5,6).
Recombinant GST-CAR was expressed in a E.coli system and purified by an affinity column in combination with FPLC chromatography
Research Use Only. CAR can be applied in in vitro transcription assays, DNA-protein and protein-protein interaction assays.
The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.
1 unit equals 1 nanogram of purified protein. 20-200ng is sufficient for an in vitro transcription assay and 100ng is sufficient for a protein-protein interaction assay.
0.5 mg/ml (in 1X dilution buffer A)
Buffer A; Tris, Glycerol, KCl, DTT, and EDTA
 
References:
1. Baes, M., et al. Mol. Cell Biol. 14: 1544-52, 1994
2. Wei, P., et al. Nature, 407; 920-23, 2000
3. Wei, P., et al. Pharmacogenomics J. 2: 117-26, 2002
4. Huang, W., et al. PNAS USA 100: 4156-61, 2003
5. Saini, S.P., et al.Mol. Pharmacol.65: 292-300, 2004
6. Stedman, C.A., et al. PNAS USA 102: 2063-2068, 2005