GST-MKP3 (DUSP6)

The mitogen-activated protein kinase phosphatases (MKPs) are dual specificity phosphatases (DUSPs) that dephosphorylate phospho-Ser/Thr and phospho-Tyr residues. At least ten mammalian MPKs have been identified (1). MKPs have been studied for their important roles in MAPK related cancer cells and innate immune response (2, 3). The MKP3, known as DUSP6 (dual specificity protein phosphatase 6), is a member of the protein tyrosine phosphatase superfamily. The MKP3 tightly regulates to ERK substrates by dephosphorylating both the phospho-Ser/Thr and phospho-Tyr residues (4). Its kinase interaction domain (KIM, residues 61-75) in addition to a conserved cytosolic domain (residues 161-177) and C-terminus domain (residues 348-381) plays an important role in ERK2 binding (5). Identification of specific activators or inhibitors of MKP3 and MPK1 may lead to the development of drug candidates against ERK pathway related diseases (3, 6-7).
Synonym:  DUSP6 and PYST1.

Recombinant GST-MKP3 is isolated from an E. coli strain that carries the coding sequence of the human MKP3 under the control of a T7 promoter.
GST-MKP3  can be applied in vitro dephosphorylation assay, DNA and protein-protein interactions assays. For Research Purposes Only.
The purified recombinant protein is greater than 90% homogeneous.
20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a DNA and protein-protein interactions assay.
2 mg/ml (in 1X dilution buffer A)
1X dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.
 
References:
1. Dickinson RJ and Keyse SM (2006) J Cell Science 119:4607-15
2. Lnag R et al (2006) J Immunol 177: 7497-7504
3. Owens DM and Keyse SM (2007) 22: 3202-12
4. Abdel-Hafiz, HA et al (1992) Mol. Endocrinol. 6: 2079-89.
5. Zhou et al (2001) J Biol Chem 276: 6506-15
6. Mark JK et al (2007) Protein Expe Purif 54: 253-60
7. Jeffrey et al (2007) Nat Rev Drug Discovery 6: 391-403