| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified PLK1 is greater than 95% homogeneous based on SDS-PAGE analysis and contains no detectable protease, DNase and RNase activity. |
| Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. |
| Applications | 1ug of PLK1 is sufficient to phosphorylate 1 ug of heat-inactivated nuclear or cytoplasmic extract at 30 C for 30 mins. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Synonym | Homo sapiens polo-like kinase 1 (Drosophila) (PLK1); PLK; STPK13. |
| Protein Sequence | MSAAVTAGKL ARAPADPGKA GVPGVAAPGA PAAAPPAKEI PEVLVDPRSR RRYVRGRFLG
KGGFAKCFEI SDADTKEVFA GKIVPKSLLL KPHQREKMSM EISIHRSLAH QHVVGFHGFF
EDNDFVFVVL ELCRRRSLLE LHKRRKALTE PEARYYLRQI VLGCQYLHRN RVIHRDLKLG
NLFLNEDLEV KIGDFGLATK VEYDGERKKT LCGTPNYIAP EVLSKKGHSF EVDVWSIGCI
MYTLLVGKPP FETSCLKETY LRIKKNEYSI PKHINPVAAS LIQKMLQTDP TARPTINELL
NDEFFTSGYI PARLPITCLT IPPRFSIAPS SLDPSNRKPL TVLNKGLENP LPERPREKEE
PVVRETGEVV DCHLSDMLQQ LHSVNASKPS ERGLVRQEEA EDPACIPIFW VSKWVDYSDK
YGLGYQLCDN SVGVLFNDST RLILYNDGDS LQYIERDGTE SYLTVSSHPN SLMKKITLLK
YFRNYMSEHL LKAGANITPR EGDELARLPY LRTWFRTRSA IILHLSNGSV QINFFQDHTK
LILCPLMAAV TYIDEKRDFR TYRLSLLEEY GCCKELASRL RYARTMVDKL LSSRSASNRL
KAS |
| Background | The human polo-like kinase (PLK) gene encodes a 603-amino acid polypeptide. By Northern blot analysis, they showed PLK was not expressed in any adult human tissues except placenta. (1) Plk localizes to the mitotic spindle, suggesting that PLK is involved in regulating mitotic spindle function. It has been observed that PLK transcripts are present at high levels in tumors of various origins and may be involved in the promotion or progression of cancers. (2) It has been proposed as a diagnostic marker for several tumors. Data supports the notion that disruption of PLK1 function could be an important application in cancer therapy. (3) |
| References | 1. Hamanaka, R. et al., Cell Growth Differ. 5: 249-257, 1994.
2. Smith, M. R.et al., Biochem. Biophys. Res. Commun. 234: 397-405, 1997.
3. Liu, X.; Erikson, R. L., Proc. Nat. Acad. Sci. 100: 5789-5794, 2003. |
| Image of SDS-PAGE |  |
