| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified MAPK14 is greater than 90% homogeneous based on SDS-PAGE analysis. |
| Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. |
| Applications | May be used in phosphorylation experiments. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Synonym | CSBP1; CSBP2; CSPB1; EXIP; Mxi2; p38; p38ALPHA; PRKM14; PRKM15; RK and SAPK2A. |
| Protein Sequence | MSQERPTFYR QELNKTIWEV PERYQNLSPV GSGAYGSVCA AFDTKTGLRV AVKKLSRPFQ
SIIHAKRTYR ELRLLKHMKH ENVIGLLDVF TPARSLEEFN DVYLVTHLMG ADLNNIVKCQ
KLTDDHVQFL IYQILRGLKY IHSADIIHRD LKPSNLAVNE DCELKILDFG LARHTDDEMT
GYVATRWYRA PEIMLNWMHY NQTVDIWSVG CIMAELLTGR TLFPGTDHIN QLQQIMRLTG
TPPAYLINRM PSHEARNYIQ SLTQMPKMNF ANVFIGANPL AVDLLEKMLV LDSDKRITAA
QALAHAYFAQ YHDPDDEPVA DPYDQSFESR DLLIDEWKSL TYDEVISFVP PPLDQEEMES |
| Background | Production of interleukin-1 and tumor necrosis factor (TNF) are inhibited by compounds called CSAIDs (cytokine-suppressive antiinflammatory drugs). 2 CSBPs (CSAID-binding proteins) have been characterized as molecular targets of pyridinyl-imidazole cytokine inhibitors. The 2 closely related targets are mitogen-activated protein kinases (MAPKs) CSBP1 and CSBP2. (1) MAPK14 is activated in response to many cellular stresses and also regulates the differentiation and/or survival of various cell types in vitro, including skeletal muscle cells and cardiomyocytes. Studies indicate that MAPK14 is required for placental organogenesis but is not essential for other aspects of mammalian embryonic development. (2)The inhibition of p38alpha MAPK blocks the release of vascular endothelial growth factor and suppresses tumor-promoted endothelial cell migration, a key step in angiogenesis.(3) |
| References | 1 Lee, J. C.; et al., Nature 372: 739-746, 1994.
2. Adams, R. H et al., Molec. Cell 6: 109-116, 2000.
3. Hendrickx,N.,et al., Biochem. Biophys. Res. Commun. 337 (3), 928-935 (2005) |
| Image of SDS-PAGE |  |
