| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified GADD45 alpha is greater than 95% homogeneous based on SDS-PAGE analysis. |
| Applications | Recombinant GADD45 alpha can be used for protein-protein interaction research. For Research use only. |
| Synonyms | Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein, DDIT-1, GADD45A, DDIT1, GADD45. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Protein Sequence | MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA
ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP
DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER |
| Background | Growth arrest and DNA Damage-Inducible Protein (GADD45A) binds both Cdks and PCNA. GADD45A is involved in DNA replication and repair. GADD45A stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. The GADD45A gene belongs to a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. GADD45A binds to proliferating cell nuclear antigen. Gadd45a binds RNA but not single- or double stranded DNA or methylated DNA in vitro. Gadd45a interacts with elongation factor 1α (EF-1α), a microtubule-severing protein that plays an important role in maintaining cytoskeletal stability. |
| References | 1. Smith M.L., et al. (1994).Science 266:1376-138 2. Chen IT., et al. (1995) Oncogene 11 (10): 1931–7. 3. Sanchez, R; et al (2010). J. Biol. Chem. 285 (29): 22196–22201. 4. Tong, T., et al (2005) MCB. 25.11.4488–4500 |
| Image of SDS-PAGE /Western-blot |  |
