| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified CAR_LBD is greater than 95% homogeneous based on SDS-PAGE analysis. |
| Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 20 units are sufficient for in vitro transcription assay and 100 units are sufficient for protein-protein interaction assays. |
| Applications | CAR-LBD can be applied in in vitro transcription assays, DNA-protein and protein-protein interaction assays. For Research Use Only. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Synonyms | CAR; CAR1; MB67; MGC150433; MGC97144; MGC97209 and NR1I3. |
| Protein Sequence | QTPVQLSKEQ EELIRTLLGA HTRHMGTMFE QFVQFRPPAH LFIHHQPLPT LAPVLPLVTH
FADINTFMVL QVIKFTKDLP VFRSLPIEDQ ISLLKGAAVE ICHIVLNTTF CLQTQNFLCG
PLRYTIEDGA RVGFQVEFLE LLFHFHGTLR KLQLQEPEYV LLAAMALFSP DRPGVTQRDE
IDQLQEEMAL TLQSYIKGQQ RRPRDRFLYA KLLGLLAELR SINEAYGYQI QHIQGLSAMM
PLLQEICS |
| Background | The constitutive androstane receptor (CAR) was identified as a member of the orphan nuclear hormone receptor family in 1994 (1). Although constitutively active, it can be further activated by `phenobarbitol-like` compounds, the most potent being the synthetic compound TCPOBOP (2). Upon activation, CAR regulates the xenobiotic drug metabolizing enzymes, cytochrome P450s (2). There are several overlapping functions between the nuclear receptors PXR and CAR (3). Recently, CAR, as well as PXR, have been shown to place a role in bile acid clearance and cholestatic liver injury (4,5,6). |
| References | 1. Baes, M., et al. (1994) Mol. Cell Biol. 14: 1544-52
2. Wei, P., et al. (2000) Nature, 407; 920-23
3. Wei, P., et al. (2002) Pharmacogenomics J. 2: 117-26
4. Huang, W., et al. (2003) PNAS USA 100: 4156-61
5. Saini, S.P., et al. (2004) Mol. Pharmacol. 65: 292-300
6. Stedman, C.A., et al. (2005) PNAS USA 102: 2063-2068 |
| Image of SDS-PAGE /Western-blot |  |
