| Purification and Quality Control | The Flag-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified Androgen Receptor is greater than 90% homogeneous based on SDS-PAGE analysis. | | Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. | | Applications | For Research Use only and not for Drug or Diagnostic purposes. AR can be used for gel mobility shift assay, for protein-protein and small molecules-protein interactions assay. | | Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. | | Synonym | AIS; DHTR; HUMARA; HYSP1; KD; NR3C4 and SBMA. | | Protein Sequence | MEVQLGLGRV YPRPPSKTYR GAFQNLFQSV REVIQNPGPR HPEAASAAPP GASLLLLQQQ
QQQQQQQQQQ QQQQQQQQQQ ETSPRQQQQQ QGEDGSPQAH RRGPTGYLVL DEEQQPSQPQ
SALECHPERG CVPEPGAAVA ASKGLPQQLP APPDEDDSAA PSTLSLLGPT FPGLSSCSAD
LKDILSEAST MQLLQQQQQE AVSEGSSSGR AREASGAPTS SKDNYLGGTS TISDNAKELC
KAVSVSMGLG VEALEHLSPG EQLRGDCMYA PLLGVPPAVR PTPCAPLAEC KGSLLDDSAG
KSTEDTAEYS PFKGGYTKGL EGESLGCSGS AAAGSSGTLE LPSTLSLYKS GALDEAAAYQ
SRDYYNFPLA LAGPPPPPPP PHPHARIKLE NPLDYGSAWA AAAAQCRYGD LASLHGAGAA
GPGSGSPSAA ASSSWHTLFT AEEGQLYGPC GGGGGGGGGG GGGGGGGGGG GGGEAGAVAP
YGYTRPPQGL AGQESDFTAP DVWYPGGMVS RVPYPSPTCV KSEMGPWMDS YSGPYGDMRL
ETARDHVLPI DYYFPPQKTC LICGDEASGC HYGALTCGSC KVFFKRAAEG KQKYLCASRN
DCTIDKFRRK NCPSCRLRKC YEAGMTLGAR KLKKLGNLKL QEEGEASSTT SPTEETTQKL
TVSHIEGYEC QPIFLNVLEA IEPGVVCAGH DNNQPDSFAA LLSSLNELGE RQLVHVVKWA
KALPGFRNLH VDDQMAVIQY SWMGLMVFAM GWRSFTNVNS RMLYFAPDLV FNEYRMHKSR
MYSQCVRMRH LSQEFGWLQI TPQEFLCMKA LLLFSIIPVD GLKNQKFFDE LRMNYIKELD
RIIACKRKNP TSCSRRFYQL TKLLDSVQPI ARELHQFTFD LLIKSHMVSV DFPEMMAEII
SVQVPKILSG KVKPIYFHTQ | | Background | The AR, androgen receptor is an androgen-activated member of the nuclear receptor superfamily of transcription factors. Signaling by the androgen receptor plays a key role in proper development and function of male reproductive organs. The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. The androgen receptor (AR) has been shown to play a critical role in the development and progression of the prostate cancer. | | References | 1. Marques,R.B., et al., Int. J. Cancer 117 (2), 221-229 (2005)
2. Metzger,E., et al., Nature 437 (7057), 436-439 (2005)
3. Murthy,S., et al., Prostate 64 (4), 362-372 (2005)
4. Hillmer,A.M., et al., Am. J. Hum. Genet. 77 (1), 140-148 (2005)
5. Gao,S., et al., Mol. Endocrinol. 19 (7), 1792-1802 (2005)
6. Chen,G., et al., Prostate 63 (4), 395-406 (2005)
7. Lim,J.T., et al., Proc. Natl. Acad. Sci. U.S.A. 102 (14), 5156-5161 (2005)
8. Kinoshita,M., et al., Am. J. Hum. Genet. 12 (4), 390-397 (2005)
9. Estebanez-Perpina,E et al., J. Biol. Chem. 280 (9), 8060-8068 (2005) |
|
