| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified P52 is greater than 95% homogeneous based on SDS-PAGE analysis. |
| Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 100 units are sufficient for protein-protein interaction assays. |
| Applications | Recombinant p52 can be utilized for in vitro function studies, including transcription, splicing, protein-DNA/RNA and protein-protein interactions. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Synonym | Homo sapiens PC4 and SFRS1 interacting protein 1 (PSIP1), transcript variant 1; DFS70; LEDGF; MGC74712; p52; p75; PAIP and PSIP2. |
| Protein Sequence | DFKPGDLIFA KMKGYPHWPA RVDEVPDGAV KPPTNKLPIF FFGTHETAFL GPKDIFPYSE
NKEKYGKPNK RKGFNEGLWE IDNNPKVKFS SQQAATKQSN ASSDVEVEEK ETSVSKEDTD
HEEKASNEDV TKAVDITTPK AARRGRKRKA EKQVETEEAG VVTTATASVN LKVSPKRGRP
AATEVKIPKP RGRPKMVKQP CPSESDIITE EDKSKKKGQE GKQPKKQPKK DEEGQKEEDK
PRKEPDKKEG KKEVESKRKN LAKTGVTSTS DSEEEGDDQE GEKKRKGGRN FQTAHRRNML
KGQHEKEAAD RKRKQEEQME TEHQTTCNLQ |
| Background | The human p52 protein is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II. The function of p52 is through interactions with transcriptional activators and the basal transcription machinery (1). In addition, p52 may also interact with several cellular proteins including the transcription coactivator PC4, the essential splicing factor ASF/SF2 and the nuclear protein nucleolin (2, 3). |
| References | 1. Ge, H. et al., (1998) EMBO J. 17, 6723-6729
2. Ge, H. et al., (1998) Molecular Cell 2, 751-759
3. Ge, H. (2000) Nucleic Acid Res. 28, e3 |
