| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified p75 is greater than 95% homogeneous based on SDS-PAGE analysis. |
| Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. 2-10 units are required for a gel mobility shift assay in a 20µl reaction; 20-50 units are sufficient for a reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay. Variable exist in different lots. |
| Applications | Recombinant p75 has been utilized for in vitro function studies, including transcription, splicing, protein-DNA/RNA and protein-protein interactions. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Synonym | Homo sapiens PC4 and SFRS1 interacting protein 1 (PSIP1), transcript variant 2; DFS70; LEDGF; MGC74712; p52; p75; PAIP and PSIP2. |
| Protein Sequence | DFKPGDLIFA KMKGYPHWPA RVDEVPDGAV KPPTNKLPIF FFGTHETAFL GPKDIFPYSE
NKEKYGKPNK RKGFNEGLWE IDNNPKVKFS SQQAATKQSN ASSDVEVEEK ETSVSKEDTD
HEEKASNEDV TKAVDITTPK AARRGRKRKA EKQVETEEAG VVTTATASVN LKVSPKRGRP
AATEVKIPKP RGRPKMVKQP CPSESDIITE EDKSKKKGQE EKQPKKQPKK DEEGQKEEDK
PRKEPDKKEG KKEVESKRKN LAKTGVTSTS DSEEEGDDQE GEKKRKGGRN FQTAHRRNML
KGQHEKEAAD RKRKQEEQME TEQQNKDEGK KPEVKKVEKK RETSMDSRLQ RIHAEIKNSL
KIDNLDVNRC IEALDELASL QVTMQQAQKH TEMITTLKKI RRFKVSQVIM EKSTMLYNKF
KNMFLVGEGD SVITQVLNKS LAEQRQHEEA NKTKDQGKKG PNKKLEKEQT GSKTLNGGSD
AQDGNQPQHN GESNEDSKDN HEASTKKKPS SEERETEISL KDSTLDN |
| Background | The human p75 protein, similar to p52, is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II in vitro through most tested activators (1, 2). Although p75 and p52 are derived from alternatively splicing of a single gene and share most coding sequence, they reveal different functions in several aspects. In addition to functioning as a transcription coactivator, p75 has been shown to be involved in growth of epithelial cells as a lens epithelial cell-derived growth factor (LEDGF), and in pathogenesis of atopic dermatitis as an autoantigen (3-5). |
| References | 1. Ge, H. et al., (1998) EMBO J. 17, 6723-6729
2. Ge, H. et al., (1998) Molecular Cell 2, 751-759
3. Singh, DP. et al., (2000) Gene 242, 265-273
4. Singh, DP. et al., (1999) Invest Ophthalmol Vis. Sci. 40, 1444-1451
5. Ochs, RL. et al., (2000) J. Allergy Clin. Immunol. 105, 1211-1220 |
| Image of SDS-PAGE /Western-blot |  |
