| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified Src1-RID is greater than 95% homogeneous based on SDS-PAGE analysis. |
| Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay. |
| Applications | Recombinant SCR1 (627-786) can be used for protein-protein interaction assays. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Synonym | bHLHe42; bHLHe74; F-SRC-1; KAT13A; MGC129719; MGC129720; RIP160; SRC1 and Homo sapiens nuclear receptor coactivator 1 (NCOA1). |
| Protein Sequence | SQTSHKLVQL LTTTAEQQLR HADIDTSCKD VLSCTGTSNS ASANSSGGSC PSSHSSLTER
HKILHRLLQE GSPSDITTLS VEPDKKDSAS TSVSVTGQVQ GNSSIKLELD ASKKKESKDH
QLLRYLLDKD EKDLRSTPNL SLDDVKVKVE KKEQMDPCNT |
| Background | Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family (1). SRC1 has been shown to be over expressed in some cancers (2). SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction (3). The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors (4,5) and have been previously used in assays detecting ligand-dependent receptor-cofactor interactions (6). This protein contains the Tobacco Etch virus (TEV) protease site and can be cleaved using acTEV to yield untagged SRC1 RID. This protein will be useful for assays where a native untagged protein is desired. |
| References | 1. Onate, S. et al., (1995) Science 270, 1354-1357
2. Yanase, T., et al., (2004) Intern. Med. 43, 368-373
3. Heery, D. et al., (1997) Nature 387, 733-736
4. Xu, J. and Li, Q., (2003) Mol. Endocrinol. 17, 1681-1692
5. Bai, Y. and Giguere, V., (2003) Mol. Endocrinol. 17, 589-599
6. Cho, M. et al., (2003) J. Biochem. Mol. Biol. 36, 207-213 |
| Image of SDS-PAGE /Western-blot |  |
