PCAF

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. PCAF belongs to the GCN5/PCAF family of nuclear HATs. The proteins consist of N-terminal and C-terminal domains separated by a deep hydrophobic cleft. The C-terminus contains the HAT domain and a bromodomain, while the N-terminus contains a PCAF-specific domain (2). Numerous studies indicate that these HATs function as histone- acetylating transcriptional co-activators (3-5). Besides histones, PCAF also acetylates non-histone proteins (2, 6).It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.
 

Source:

The PCAF was expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography.
Research Use Only.

Applications: 

Recombinant PCAF can be used 1) for protein-protein interaction assay; 2) for in vitro transcription assay; 3) for in vitro acetylation assay; and 4) for cell growth assay.

Quality Control:

Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase and RNase activity.

Unit Definition:

1 unit equals 1 nanogram of purified protein. 1-5 units are sufficient for a gel mobility shift assay in a 20µl reaction; 50-100 units are sufficient for reconstituted transcription assay and 100-200 units are sufficient for a protein-protein interaction assay or an acetylation assay.

Concentration:

Reagents Supplied:

1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.

References:
1. Yang, X-J (2004) Nuc Acids Res, 32, (3) 959-976
2. Sterner, D.E. and Berger, S.L (2000) Microbiol and Mol Biol Rev, 64 (2) 435-459
3. Roth,S.Y. et al., (2001) Annu. Rev. Biochem., 70, 81–120
4. Carrozza,M.J., et al., (2003) Trends Genet., 19, 321–329
5. Nakatani,Y. (2001) Genes Cell, 6, 79–86
6. Kouzarides,T. (2000) EMBO J., 19, 1176–1179