| Description | Cyclophilins catalyze the isomerization of peptide bonds from trans form to cis form at proline residues and accelerate the folding of proteins. Human cyclophilin A forms a dimer, and inhibits the calcium/calmodulin-dependent phosphatase calcineurin. This inhibition is thought to suppress organ rejection by halting the production of the pro-inflammatory molecules TNF alpha and interleukin 2.
Cyclophilin-A can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. | | Synonym | Peptidylprolyl isomerase A, CYPA, CYPH, MGC12404, MGC23397, MGC117158, PPIase A, Rotamase A, PPIA, Peptidyl-prolyl cis-trans isomerase A, EC 5.2.1.8, Cyclophilin A, Cyclosporin A-binding protein. | | Sequence | MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALSTGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSMANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIADCGQLE | | Formulation | 1 mg/ml solution containing 20mM Tris-HCl pH7.9, 100mM NaCl, 0.5mM EDTA, 1mM DTT and 20% glycerol. | | Storage and Stability | Recombinant human Cyclophilin A although stable at 4°C for 1 week, should be aliquot and stored below -20°C. Avoid freeze thaw cycles. | | Applications | Specific activity is > 200 nmoles/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute in Tris-Hcl pH8.0 using chymotrypsin. For research use only. | | SDS-PAGE /Western-blot |  |
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