| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified SRC1-RID is greater than 90% homogeneous based on SDS-PAGE analysis. |
| Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay. |
| Applications | Recombinant His tagged SCR1 (627-786) can be used for protein-protein interaction assays. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Synonym | bHLHe42; bHLHe74; F-SRC-1; KAT13A; MGC129719; MGC129720; RIP160; SRC1 and Homo sapiens nuclear receptor coactivator 1 (NCOA1). |
| Protein Sequence | SQTSHKLVQL LTTTAEQQLR HADIDTSCKD VLSCTGTSNS ASANSSGGSC PSSHSSLTER
HKILHRLLQE GSPSDITTLS VEPDKKDSAS TSVSVTGQVQ GNSSIKLELD ASKKKESKDH
QLLRYLLDKD EKDLRSTPNL SLDDVKVKVE KKEQMDPCNT |
| Background | Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family (1). SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction (2). The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors (3,4) and has been previously used in assays detecting ligand-dependent receptor-cofactor interactions (5). |
| References | 1. Onate, S. et al., (1995) Science 270, 1354-1357
2. Heery, D. et al., (1997) Nature 387, 733-736
3. Xu, J. and Li, Q., (2003) Mol. Endocrinol. 17, 1681-1692
4. Bai, Y. and Giguere, V., (2003) Mol. Endocrinol. 17, 589-599
5. Cho, M. et al., (2003) J. Biochem. Mol. Biol. 36, 207-213 |
