SC35 (SFRS2 or SRp30b) Pre-mRNA Splicing Factor

SC35, a member of the SR protein family, is an essential pre-mRNA splicing factor and can function as an essential splicing factor as well as an alternative splicing factor (1, 2). Phosphorylation of serine residues located within the SR domain directly regulates SC35 activity and compartmentalization of other SR splicing factors (3, 4). In addition to interacting with RNA and other splicing factors, SC35 has been shown to interact either directly or indirectly with the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, thereby suggesting a potential role of SC35 in coordinating transcription and pre-mRNA splicing (5, 6).
The human SC35 wild type protein was expressed in a baculovirus system and purified by affinity and FPLC chromatography.
Recombinant SC35 protein can be used for: 1) in vitro function studies including pre-mRNA splicing, cross linking and other RNA binding assays; 2) protein-protein interaction assay; and 3) cell growth and proliferation assays.
The purified protein is greater than 95% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 1-5 units are the amount sufficient for a gel mobility shift assay in a 20 µl reaction, 100 units are sufficient for a protein-protein interaction assay and 100-500 units are required for a splicing assay in a 20 μl reaction.
variable in different lots
1x buffer B: 20 mM HEPES-Na (pH 7.9), 20% Glycerol, 42 mM (NH4)2SO4, 0.5 mM DTT and 0.2 mM EDTA
 
References:
1. Fu, X.D., et al., (1992) Science 256, 535-538
2. Zahler, A.M., et al., (1992) Genes & Dev. 6, 837-847
3. Gui, J.F., et al., (1994) Proc. Natl. Acad. Sci. USA 91, 10824-10828
4. Colwill, K., et al., (1996) EMBO J. 15, 265-275
5. Mortillaro, M.J., et al., (1997) Proc. Natl. Acad. Sci. USA 93, 8253-8257
6. Misteli, T., et al., (1997) Nature 387, 523-527