| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified USF1 is greater than 90% homogeneous based on SDS-PAGE analysis. |
| Unit Definition (Activity) | 1 unit equals 1 nanogram of purified protein. 5-50 units are sufficient for DNA binding assays. 50-500 units are required for an in vitro transcription assay and 100 units are required for protein-protein interaction assay. |
| Applications | Recombinant USF1 can be used for: 1) in vitro transcription assays; 2) DNA binding assays (gel mobility shift assys and DNA footprint assays), and 3) protein-protein interaction assays. |
| Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. |
| Synonym | bHLHb11; FCHL; FCHL1; HYPLIP1; MLTF; MLTFI and UEF. |
| Protein Sequence | MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV FRTENGGQVM
YRVIQVSEGQ LDGQTEGTGA ISGYPATQSM TQAVIQGAFT SDDAVDTEGT AAETHYTYFP
STAVGDGAGG TTSGSTAAVV TTQGSEALLG QATPPGTGQF FVMMSPQEVL QGGSQRSIAP
RTHPYSPKSE APRTTRDEKR RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK
GGILSKACDY IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG
LEVVIKNDSN |
| Background | Human Upstream Stimulatory Factor 1 (USF1) regulates the transcription of many genes involved in lipid and glucose homeostasis and co-localizes with familial combined hyperlipidemia (FCHL) and type 2 diabetes on chromosome 1q22-23. (1-3). The USF1 protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. It is a basic helix-loop-helix leucine zipper (b-HLH-ZIP) protein that shares a common DNA-binding specificity with the c-Myc oncoproteins (4-6). |
| References | 1.Putt, W. et al., (2004) Hum. Mol. Genet. 13, 1587-1597.
2.Pajukanta, P. et al., (2004) Nat. Genetics 36, 371-376.
3.Du, H. et al., (1993) EMBO J. 12, 501-511.
4.Gregor, PD. Et al., (1990) Genes & Dev. 4, 1730-1740.
5.Ge, Y. et al., (2003) J. Biol. Chem. 278, 49901-49910.
6.Sawadogo, M. et al., (1985) Cell 43, 165-175. |
