TBP (TATA box Binding Protein)

The TATA-binding protein (TBP) is believed to function as an essential factor of the general transcription machinery and to be involved in transcription by all three eukaryotic RNA polymerases (pol I, II, and III).
TBP specifically binds to the TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor suppressor proteins (1-3).
The His tagged recombinant TBP is isolated from an E. coli strain that carries the coding sequence for human TBP under the control off T7 promoter (3).
TBP can be used for 1) in vitro transcription; 2) gel mobility shift assay in the presence of double-stranded oligonucleotide containing a TATA element; 3) for in vitro footprinting assay in the presence of TATA-containing DNA fragment (1, 4) and 4) for protein-protein interactions assay.
Protein is greater than 95% homogeneous based on SDS-PAGE assay analysis.
1 unit equals 1 nanogram of purified protein. 1 unit is the amount sufficient for a gel mobility shift assay in a 20 µl reaction. 10 units are sufficient for a 25 µl reconstituted transcription reaction and 100 units are sufficient for a protein-protein interaction assay detected by immuno-blot system.
May vary in different lots.
1x dilution buffer A: 20 mM Tris-Cl (pH 7.9) 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
 
References:
1. Horikoshi, M. et al., (1988) Cell 54, 1033-1042
2. Hernandez, N. (1993) Genes & Dev. 7, 1291-1308
3. Holffman, A. et al., (1991) Nucleic Acids Res. 19, 6337-6338
4. Ge, H. et al., (1994) J. Biol. Chem. 269, 17136-17140
5. Grace et al., (2004) J. Bio Chem, 279:8102-8110
6. Francois et al., (2005) J. Virology, 79:17, 11082-11094,