Package of co-activators (p52, P75)
Description: Package of coactivators include p52(gene access number: NM_021144, Cat# p1029-01, 10ug), p75(gene access number: NM_033222, Cat# p1030-01, 10ug) and C terminal domain of p75 (Cat# p1061-01, 10ug). View Full Specifications		SKU Price G517 $851.00 Quantity:
The human p52 protein is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II. The function of p52 is through interactions with transcriptional activators and the basal transcription machinery (1). In addition, p52 may also interact with several cellular proteins including the transcription coactivator PC4, the essential splicing factor ASF/SF2 and the nuclear protein nucleolin (2, 3). Recombinant His tagged p52 protein (wild type, 333 amino acids) is isolated from an E. coli system.  Recombinant p52 can be utilized for in vitro function studies, including transcription, splicing, protein-DNA/RNA and protein-protein interactions.The human p75 protein, similar to p52, is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II in vitro through most tested activators (1, 2). Although p75 and p52 are derived from alternatively splicing of a single gene and share most coding sequence, they reveal different functions in several aspects. In addition to functioning as a transcription coactivator, p75 has been shown to be involved in growth of epithelial cells as a lens epithelial cell-derived growth factor (LEDGF), and in pathogenesis of atopic dermatitis as an autoantigen (3-5). Recombinant His tagged p75 protein (wild type, 530 amino acids) is expressed and isolated from an E. coli strain.Recombinant p75 has been utilized for in vitro function studies, including transcription, splicing, protein-DNA/RNA and protein-protein interaction.Protein is greater than 95% homogeneous based on SDS-PAGE analysis. 1 unit equals 1 nanogram of purified protein. 2-10 units are required for a gel mobility shift assay in a 20µl reaction; 20-50 units are sufficient for a reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay. variable in different lots 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA   References: 1. Ge, H. et al., (1998) EMBO J. 17, 6723-6729 2. Ge, H. et al., (1998) Molecular Cell 2, 751-759 3. Singh, DP. et al., (2000) Gene 242, 265-273 4. Singh, DP. et al., (1999) Invest Ophthalmol Vis. Sci. 40, 1444-1451 5. Ochs, RL. et al., (2000) J. Allergy Clin. Immunol. 105, 1211-1220

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