E2F-1 (RBAP-1) Transcription Factor and Rb-mediator

E2F was originally identified as a transcription factor mediating the transcription of adenovirus E2 gene. It is a heterodimer composed of two structurally related subunits, termed E2F and DP. E2F is encoded by at least five genes, E2F-1 through E2F-5, and DP is encoded by at least two genes, DP-1 and DP-2. All these genes (E2F-1 through E2F-5, DP-1 and DP-2) have been considered as members of the E2F gene family because they have a number of common structural and functional characteristics (1-4). Among those members of the E2F family, E2F-1 was the first E2F gene cloned and characterized. The E2F-1 protein has been shown to interact with RB both in vitro and in vivo (5, 6). Although the E2F proteins can function as transcription factors by themselves, they should be considered as RB-mediators as well because almost every functional response of RB protein(s) requires the presence of E2F protein(s).

Applications: Recombinant E2F-1 protein can be used 1) for in vitro function studies including transcription, DNA binding and DNA bending assays; 2) for protein-protein interaction assay; and 3) for cell growth and proliferation assays.

Quality: The purified protein is greater than 95% homogeneous based on SDS-PAGE analysis.

Supplied in 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA

References:

1. Bagchi, S. et al., (1990) Cell 62, 659-669.
2. Helin, K. et al., (1992) Cell 70, 337-350.
3. Kaelin, WG. et al., (1992) Cell 70, 351-364.
4. Shan, B. et al., (1992) Mol. Cell. Biol. 12, 5620-5631.
5. Chellappan, SP. et al., (1991) Cell 65, 1053-1061.
6. Schwarz, JK. et al., (1993) EMBO J. 12, 1013-1020.