| Purification and Quality Control | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified Brg1 is greater than 90% homogeneous based on SDS-PAGE analysis. | | Unit Definition (Activity) | 1 unit equals 1 nanogram purified protein. 50 - 100 units are required for an in vitro nucleosome remodeling assay and 100 units are required for a protein-protein interaction assay. | | Applications | Recombinant BRG1 can be used 1) for protein-protein interaction assay; 2) for in vitro transcription assay; 3) for in vitro nucleosome remodeling assay; and 4) for cell growth assay. | | Formulation and Storage | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles. | | Synonym | Brahma-related Gene 1; BAF190; BRG1; FLJ39786; hSNF2b; RTPS2;SNF2; SNF2-BETA; SNF2L4; SNF2LB and SWI2. | | Background | The wild type human brahma-related gene 1 (Brg1) encodes a protein of 1,647 amino acids that contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription regulation (1-3). BRG1 is an essential component of the SWI/SNF chromatin remodeling complexes and implicated in multiple functions through its interaction with different proteins, including the tumor suppressor protein pRb (Cat#P2007) (4), serine-threonine kinase LKB1 (5), and other transcription factors (6,7). Although Brg1 is involved in chromatin remodeling as a complex with other SWI/SNF proteins, purified BRG1 itself is capable of remodeling mono-nucleosomes and nucleosomal arrays in vitro (8). Mutations of Brg1 have been found in multiple tumor cell lines (9). | | References | 1. Khavari, P.A. et al., (1993) Nature 366, 170-174
2. Fryer, C.J. et al., (1998) Nature 393, 88-91
3. Sif, S. et al., (1998) Genes Dev. 12, 2842-2851
4. Dunaief, J.L. et al., (1994) Cell 79, 119-130
5. Marignani, P.A. et al., (2001) J. Biol. Chem. 276, 32415-32418
6. Brockmann, D. et al., (2001) Gene 277, 111-120
7. Barker, N. et al., (2001) EMBO J. 20, 4935-4943
8. Phelan, M.L. et al., (1999) Mol. Cell 3, 247-253
9. Wong, A.K. et al., (2000) Cancer Res. 60, 6171-6177 |
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