| Description | HiExpressionTM transfection reagents is formulated with proprietary cationic polymer to ensure low cytotoxicity and high transfection efficiency. HiExpressionTM transfection reagents was shown to efficiently deliver genes to various established cell lines as well as primary cells, which include HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines with low cytotoxicity. HiExpressionTM transfection reagents, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates, or 150 to 300 transfections in 6 well plates, or 13 transfections in 150 mM plates. It can be used for recombinant mammalian protein expression. |
| Advantges | High transfection efficiency Low cytotoxicity Serum tolerate High expression Simple protocol Compatible price Suitable for high-throughput application Efficient for difficult-to-transfect cells Transfect a broad range of mammalian cells |
Transfection Efficiency | Transfection efficiency of HiExpressionTM transfection reagents (#3) with two other popular transfection reagents (#1, #2). Amount of HiExpressionTM transfection reagents: 3 µl; 1 µg GFP expression plasmid DNA; 293T cells grown on a 24-well plate.  |
Expression Efficiency | Transfection efficiency of HiExpressionTM transfection reagents (#3) with two other popular transfection reagents (#1, #2). GFP protein expression level was analyzed with fluorescence reading in SpectroMax M5. 
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| Expression test by Western-blot | One 86 kDa protein expression plasmid was transfected into 293T cells by three different transfection reagents. HiExpressionTM transfection reagent is #3 and two other popular transfection reagents are #1 and #2. Cells were collected after 24 and 48 hours, target protein expression levels were confirmed by western-blot.  |
| Storage and stability | Upon arrival store this product at -20 oC. If stored properly, the product will be stable for 12 months or longer. |
| Protocol | Please contact us at info@proteinone.com or call us: 301-738-0782. |
