The Active Protein Array (APA) platforms are unique protein arrays designed for the multiplex detection and interaction assessment of multiple human activated regulatory whole proteins with DNA, RNA, and/or ligands of interest. This unprecedented format sets a new standard in high throughput protein analysis.

The Active Protein Array platform consist of three main array templates:
  • NEW Transcription Array - Catalog #8001-01
    36 Activated Transcription factors, Kit contains 2 arrays and reagents.
  • Nuclear Receptor Array - Recombinant nuclear receptors
  • Cancer Array - Tumor suppressor & onco-proteins

    Platform for Protein-Protein, Protein-DNA, and Protein-Ligand Interactions



    Sample Processing
    The Active Protein Array system is fast and easy. Each array consists of active, highly purified proteins spotted in triplicate on a special nitrocellulose membrane. A protein, DNA, or ligand of interest is used as a probe to search for interactions with the immobilized proteins. These interactions are assessed either by tagging the probe with a small molecule or radioactivity, or by using an antibody to the probe. Currently the signals are easily visualized by radioactive detection.

    Benefits
  • Simultaneous screening of interactions with multiple human regulatory proteins in a single experiment
  • Fast and easy alternative to pull-down assays, the yeast two-hybrid system, and co-imunoprecipitation
  • Versatile design allows for different detection methods to be used depending on the specific needs of the user
  • Arrayed proteins are readily available from ProteinOne in an active and highly purified form for further experimentation


    ORDERING INFORMATION
    Active Protein Array

    Catalog Part Name Description
    8001-01 Transcription Array 2 Nitrocellulose Membrane arrays
    8001-02 Transcription Array 4 Nitrocellulose Membrane arrays
    8001-03 Transcription Array 2 Nitrocellulose Glass Slide arrays
    8001-04 Transcription Array 4 Nitrocellulose Glass Slide arrays


    PDF Users Manual - Download



    For Research Use Only.
    Not for use in diagnostic procedures.

    Leading Advancement in Protein ScienceTM

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