SERVICES
Monoclonal Antibody Production
We offer an array of services for the research, development and production of monoclonal antibodies.
Please contact us for a quote or further information:
Call at (301) 738-0782
Or e-mail at info@proteinone.com
Polyclonal Antibody Production
Protein One offers custom polyclonal development, production and purification services. We offer an array of services for the research, development and production of polyclonal antibodies.
Polyclonal antibody production One rabbit
- Day 0 High purity antigen injection (250 ug)
- Day 21 Antigen injection (125 ug)
- Day 31 First bleeding (20 ml)
- Day 42 Antigen injection (125 ug)
- Day 52 Second bleeding (~20 ml)
- Day 63 Antigen injection (125 ug)
- Day 77 Final bleed (~90 ml)
- 1 week ELISA titration, western-blot confirm
- 1 week IgG purification, western-blot confirm
Please contact us for a quote or further information:
Call at (301) 738-0782
Or e-mail at info@proteinone.com
E. Coli System
For protein expression and purification in E.coli, customer has five different tags for selecting: GST, MBP, His, Flag, biotin. Customer could choose N terminal or C terminal insert of the tag. We also provide tag remove and endotoxin removal services.
- Cloning
- Subcloning
- Generation of bacmid DNA
- Multiprotein Complex Expressions
- Transfection
- Tittering and amplification of virus
- Optimization of the expression conditions
- Mini- or large-scale infection
- Conventional purification
- Affinity purification
- Dialysis in a proper buffer and measure of concentration
- SDS-PAGE and Western blot analysis
- Protocol optimization
Benefits
Time Savings: Reduce turn around time associated with pre-clinical development. Extend the capabilities of your research.
Excellence: Our protein expression and purification experience ensures product quality and accuracy.
Cost: Custom service, rapid turnaround at cost effective prices.
Protein One Capabilities
With protein production and purification capacities from 1mg to over 100mg, Protein One can accommodate projects ranging in size from bench top to bioreactor. From target discovery through pre-clinical development and beyond, Protein One is the best source for high quality protein production, outstanding service, and reputation that has made Protein One a market leader.
Please contact us for a quote or further information:
Call at (301) 738-0782
Or e-mail at info@proteinone.com
Yeast Expression System
- Cloning
- Subcloning
- Generation of bacmid DNA
- Multiprotein Complex Expressions
- Transfection
- Tittering and amplification of virus
- Optimization of the expression conditions
- Mini- or large-scale infection
- Conventional purification
- Affinity purification
- Dialysis in a proper buffer and measure of concentration
- SDS-PAGE and Western blot analysis
- Protocol optimization
Benefits
- Time Savings: Reduce turn around time associated with pre-clinical development. Extend the capabilities of your research.
- Excellence: Our protein expression and purification experience ensures product quality and accuracy.
- Cost: Custom service, rapid turnaround at cost effective prices.
Protein One Capabilities
With protein production and purification capacities from 1mg to over 100mg, Protein One can accommodate projects ranging in size from bench top to bioreactor. From target discovery through pre-clinical development and beyond, Protein One is the best source for high quality protein production, outstanding service, and reputation that has made Protein One a market leader.
Please contact us for a quote or further information:
Call at (301) 738-0782
Or e-mail at info@proteinone.com
Baculovirus System
Our Custom Protein Purification Baculovirus offers all aspects of protein expression, production, purification, and development, from gene to protein including:
- Cloning
- Subcloning
- Generation of bacmid DNA
- Multiprotein Complex Expressions
- Transfection
- Tittering and amplification of virus
- Optimization of the expression conditions
- Mini- or large-scale infection
- Conventional purification
- Affinity purification
- Dialysis in a proper buffer and measure of concentration
- SDS-PAGE and Western blot analysis
- Protocol optimization
Benefits
- Time Savings: Reduce turn around time associated with pre-clinical development. Extend the capabilities of your research.
- Excellence: Our protein expression and purification experience ensures product quality and accuracy.
- Cost: Custom service, rapid turnaround at cost effective prices.
Protein One Capabilities
With protein production and purification capacities from 1mg to over 100mg, Protein One can accommodate projects ranging in size from bench top to bioreactor. From target discovery through pre-clinical development and beyond, Protein One is the best source for high quality protein production, outstanding service, and reputation that has made Protein One a market leader.
Please contact us for a quote or further information:
Call at (301) 738-0782
Or e-mail at info@proteinone.com
Mammalian Transient Expression System
- Cloning
- Subcloning
- Generation of bacmid DNA
- Multiprotein Complex Expressions
- Transfection
- Tittering and amplification of virus
- Optimization of the expression conditions
- Mini- or large-scale infection
- Conventional purification
- Affinity purification
- Dialysis in a proper buffer and measure of concentration
- SDS-PAGE and Western blot analysis
- Protocol optimization
Benefits
- Time Savings: Reduce turn around time associated with pre-clinical development. Extend the capabilities of your research.
- Excellence: Our protein expression and purification experience ensures product quality and accuracy.
- Cost: Custom service, rapid turnaround at cost effective prices.
Protein One Capabilities
With protein production and purification capacities from 1mg to over 100mg, Protein One can accommodate projects ranging in size from bench top to bioreactor. From target discovery through pre-clinical development and beyond, Protein One is the best source for high quality protein production, outstanding service, and reputation that has made Protein One a market leader.
Please contact us for a quote or further information:
Call at (301) 738-0782
Or e-mail at info@proteinone.com
Custom Peptide
Peptide length: up to 100 mers in length
Peptide scale: mg to grams
Purity scale: crude, de-salted, >75%, >85%, >90%, >95%, >98%, >99%.
Regular modifications: N-acetylation, C-amidation, fatty acid, etc.
Uncommon amino acids and amino acid derivatives
Unusual modifications: biotinylation, fluorescence/dye labeling, phosphorylation and sulfation, cyclic peptides (via disulfide bond or amide bond), methylated peptides, heavy isotope labeled peptides, peptoids and peptides for click chemistry.
Please contact us for a quote or further information:
Call at (301) 738-0782
Or e-mail at info@proteinone.com
