GADD45 alpha

• Species; Human
• Expression Host: E.coli
• Tag: His-tag
• Purity: 95%
• Molecular Weight: 19.4 kDa.
• Gene Accession Number: NM_001924.

Purification and Quality Control
The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified GADD45 alpha is greater than 95% homogeneous based on SDS-PAGE analysis.

Applications
Recombinant GADD45 alpha can be used for protein-protein interaction research.

Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym
Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein, DDIT-1, GADD45A, DDIT1, GADD45.

Protein Sequence
MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA
ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP
DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER

Background
Growth arrest and DNA Damage-Inducible Protein (GADD45A) binds both Cdks and PCNA. GADD45A is involved in DNA replication and repair. GADD45A stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. The GADD45A gene belongs to a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. GADD45A binds to proliferating cell nuclear antigen. Gadd45a binds RNA but not single- or double stranded DNA or methylated DNA in vitro. Gadd45a interacts with elongation factor 1α (EF-1α), a microtubule-severing protein that plays an important role in maintaining cytoskeletal stability.

Image of SDS-PAGE /Western-blot