GST-Tagged Steroid Receptor Coactivator 1-Receptor Interaction Domain (627-786)

• Species: Human
• Expression: Host E.coli
• Tag: GST-tag
• Purity: 90%
• Molecular Weight: 44.0 kDa.
• Gene Accession Number: NM_003743.

Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivoator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family (1). SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction (2). The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors (3,4) and has been previously used in assays detecting ligand-dependent receptor-cofactor interactions (5).

The wild type human GST-SRC1 (amino acids 627-786) was expressed in e.Coli and purified by an affinity column.
Research Use Only and not for Diagnostic purposes. Recombinant GST-SCR1 (627-786) can be used for protein-protein interaction assays.

Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase and RNase activity.
1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay.

0.5 mg/ml (in 1X dilution buffer A).GST-SRC1-RiD
Buffer A: Tris-Cl , Glycerol, KCl, EDTA and DTT