GST-tagged Transcription Factor IIE subunit p56

• Species: Human
• Expression: Host E.coli
• Tag: GST-tag
• Purity: 90%
• Molecular Weight: 76.2 kDa.
• Gene Accession Number: NM_005513.

The human Transcription Factor IIE (TFIIE) is composed of 56 kDa (α) and 34 kDa (α) subunits and is shown to be a heterotetramer (1, 2). The 56-kDa subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain (3). TFIIE binds to RNA polymerase II in solution and joins the preinitiation complex probably concomitant with RNA polymerase II and TFIIF (4).

Gene synonym include: Homo sapiens general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1); FE; TF2E1 and TFIIE-A.

The fusion protein is purified from an E. coli strain that contains the coding sequence of human TFIIE α subunit under the control of a T7 promoter (5).
GST-TFIIEα has been used for protein-protein interactions assay (6).
The purified protein is greater than 95% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 100 units (ng) are sufficient for a protein-protein interaction assay.
variable in different lots
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA

References:
1. Ohkuma, Y. et al., (1990) Proc. Natl. Acad. Sci. USA 87, 9163-9167
2. Inostroza, J. et al., (1991) J. Biol. Chem. 266, 9304-9308
3. Peterson, M. et al., (1991) Nature 354, 369-373
4. Flores, O. et al., (1990) J. Biol. Chem. 265, 5629-5634
5. Maldonado, E. et al., (1996) Methods Enzymol. 274, 72-100
6. Kaludov, N., et al., (2000) Nucleic Acids Res. 28, 1921-1928

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.