MAD (MAX dimerization protein 1, MXD1)

• Species: Human
• Expression Host: E.coli
• Tag: Flag-tag
• Purity: 90%
• Molecular Weight: 26.6 kDa.
• Gene Accession Number: NM_002357.

Purification and Quality Control
The Flag-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified MAD is greater than 90% homogeneous based on SDS-PAGE analysis.

Unit Definition (Activity)
1 unit equals 1 nanogram of purified protein.

Applications
MAD can be applied to DNA binding, proliferation, differentiation, and apoptosis research studies.

Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym
MXD1; MAD1; MGC104659.

Protein Sequence
MAAAVRMNIQ MLLEAADYLE RREREAEHGY ASMLPYNNKD RDALKRRNKS KKNNSSSRST
HNEMEKNRRA HLRLCLEKLK GLVPLGPESS RHTTLSLLTK AKLHIKKLED CDRKAVHQID
QLQREQRHLK RQLEKLGIER IRMDSIGSTV SSERSDSDRE EIDVDVESTD YLTGDLDWSS
SSVSDSDERG SMQSLGSDEG YSSTSIKRIK LQDSHKACLG L

Background
MAX dimerization protein belongs to a subfamily of MAX-interacting proteins. This protein competes with MYC for binding to MAX to form a sequence-specific DNA-binding complex, acting as a transcriptional repressor (while MYC appears to function as an activator) and is a candidate tumor suppressor. Both Myc and Mad, as well as the more recently described Mnt and Mga proteins, form heterodimers with Max, permitting binding to specific DNA sequences. These DNA-bound heterodimers recruit coactivator or corepressor complexes that generate alterations in chromatin structure, which in turn modulate transcription (1). The wild-type c-Myc and c-Myc/MadBR proteins have indistinguishable biological activity and target gene recognition in vivo (2).

References:
1. Grandori,C., et al. Annu. Rev. Cell Dev. Biol. 16, 653-699 (2000)
2. Nikiforov,M.A., et al. J. Biol. Chem. 278 (13), 11094-11099 (2003)

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DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.